Fig 6. Estrogen treatment increases neutrophil percentages in NSP^-/- knock out mice.

Red blood cell-depleted whole splenocytes (A), peripheral blood (B), and bone marrow (C) cells from placebo- and estrogen-treated NSP^-/- knockout mice were stained with neutrophil surface markers as described in Fig 3. The representative FACS plots are shown. The summary graphs show the percentages of neutrophils (CD11b⁺GR1⁺) in the spleen, blood, and bone marrow cells from placebo- and estrogen-treated NSP^-/- mice (means ± SEMs, n≥5). Unpaired student t test (placebo vs estrogen) were preformed; ***, p < 0.001.