Fig 8. Gene signatures identified rSm-p80 + ODN immunized mice in an independent blinded study.

Ten mice were blindly immunized with either rSm-p80 + ODN or ODN alone. One-day post-immunization spleens were dissected and RNA isolated to prepare cDNA. Quantitative RT-PCR expression levels of selected genes identified with 80% accuracy the immunization given for each mouse. Relative fold change of CSF2, HEBP1, S100A8, STAT1, and TLR4 for each mouse. Values represent the average of triplicate PCR experiments normalized to corresponding expression value of each gene and GAPDH from a naïve mouse. Cutoff value for each gene was assigned based on top 50 percentile for each gene; mice were assigned as control (blue) or experimental (red). The overall gene pattern of the 5 genes was used to predict each mouse as control or experimental; 8 out of 10 mice were correctly identified.