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. 2017 Feb 13;12(2):e0172227. doi: 10.1371/journal.pone.0172227

Fig 4. tmem88a cDNA from tmem88a mutants is not digested by ScaI.

Fig 4

(A) Schematic representation of the tmem88a (t88a) mRNA transcript. Non-coding and coding exons are shown as white and black blocks respectively. Introns as depicted as solid lines. The deleted site in tmem88aΔ8 mutants is shown by a red bar. F and R primers used for amplification of cDNA are shown as arrows. (B) 2% agarose gel showing ScaI digestion of wild type PCR product into fragments of 244 and 179 bp in size. Tmem88a mutant cDNA is not digested by ScaI.