. 2017 Feb 13;12(2):e0172227. doi: 10.1371/journal.pone.0172227

Table 1. Sequences of primers used for qRT-PCR.

	Forward primer (5′-3′)	Reverse primer (5′-3′)
fli1a	`AGCGCTACGCCTACAAGTTC`	`AGCTCCAGTATGGGGTTGTG`
gata1a	`CTCGTTGGGTGTCCCCCGGT`	`CGACGAGGCTCGGCTCTGGA`
hbbe1	`AACTGTGCTCAAGGGTCTGG`	`TACGTGGAGCTTCTCGGAGT`
lyz	`GCACGGCCTACTGGGAAAGCA`	`CCCAGGGGTCCCGTCATCACA`
myoD	`GGGCCCAACGTGTCAGACGA`	`GTTGAGGGCAGCTGGTCGGG`
nkx2.5	`CTGTGCCAGTTTTGGTTCGG`	`CGCAGGGTAGGTGTTGTAGG`
runx1	`AGTGGACGGACCCCGAGAGC`	`ACCGCATGGCACTTCGCCTC`
scl	`CAACGATGGTTCGCAGCCCA`	`ACCGCCGACCATGTCGTCCT`
spi1b	`ATGCGGCCAGTGTGCATCGC`	`CACCGATGTCCGGGGCAAGT`
tmem88a F1R1	`CCTGCCATCGCTCGTCATGGT`	`AGACGGCACGGCTGTATGGGA`
tmem88a F2R2	`TGCCAGTCTGAGGAATCTGCC`	`GTTTGCTGGAGTACTGGAGGAGA`
tmem88b	`CCTCCACCTTACTCCCCAGA`	`ATGGGATAAAGCACAGCCCC`

fli1a, friend leukaemia integration 1a; gata1a, GATA-binding factor 1a; hbbe1, haemoglobin beta-embryonic 1; lyz, lysozyme; runx1, runt-related transcription factor 1; scl, stem cell leukaemia; spi1, spleen focus forming virus (sffv) and proviral integration oncogene 1b (also known as pu.1).