Figure 1. The NTR of ISWI contains several conserved sequence motifs.
(A) Schematic representation of the ISWI domain composition. The grey inset shows the sequence and motifs of the NTR. Arrows indicate amino acids within the NTR of Drosophila ISWI that crosslinked to Lobe 2 of the ATPase domain (Table 1). HSS, HAND-SANT-SLIDE domain. (B–E) Sequence logos showing the sequence conservation of (B) ppHSA, (C) AT-hook, (D) AutoN, and (E) AcidicN. X-Axis values are amino acid positions in D. melanogaster ISWI. See Figure 1—figure supplement 1 for full alignment. (F) Alignment of the ppHSA motif of Drosophila (Dm) ISWI with the human (Hs) ISWI homologs SNF2H and SNF2L and representatives of unrelated remodeler families.
Figure 1—figure supplement 1. Alignment of ISWI homologs from various organisms.
Search for homologous proteins and alignment was done using HHblits (toolkit.tuebingen.mpg.de/hhblits). 26 Sequences lacking an NTR were manually deleted from the alignment.