Table 2. The EST based SSR markers identified and developed.

Primer Name	Sequence	Repeat motif	Expected product size	Gene from which marker developed	GenBank Accession number	Length of nucleotides	Annealing temperature
EG01F	5′TGCCTTGTCTCTCCAGAAAACT3′	(AAAG)3	384	Arabidopsis thaliana chromosome 4		22	60.415
EG01R	5′CACTGATGCACATGAAACTGAA3′					22	59.764
EG02F	5′TCTCTTCTCGAAACTAAGCATGG3′	(CTT)4	397	do		23	60.028
EG02R	5′CATCCCGTGTACTATCTCCCTC3′					22	59.848
EG03F	5′CCCGAGAAAGTTGGATACAGAA3′	(T)10	279	do		22	60.474
EG03R	5′AACGTCACTTGTCGATTTGTTG3′					22	60.074
EG04F	5′TGAAATCGAAAACGCGCAGA3′			Arabidopsis thaliana agamous-like MADS-box protein AGL11 mRNA	NM_117064.6	20	59
EG04R	5′CCAGATCCAGAACCAGCAGT3′					20	59
EG05F	5′GTGTCCGATCCAAGAAGCAC3′			Arabidopsis thaliana agamous-like MADS-box protein AGL5 mRNA	NM_180046.2	20	60
EG05R	5′CCCGACTGGTGAGAAGAAGT3′					20	60
EG06F	5′CAATGGAGGAAGGTGGGAGT3′			Arabidopsis thaliana agamous-like MADS-box protein AGL1 mRNA	NM_115740.2	20	59
EG06R	5′CGGCATCACACAAGACAGAG3′					20	59
EG07F	5′GGAATTTGCGGTGTTGGACT3′			Elaeis guineensis MADS-box transcription factor 21-like (LOC105034563), mRNA	XM_010909778.1	20	59
EG07R	5′GGCAACCTCAGCATCACAAA3′					20	59
EG08F	5′GGAATTTGCGGTGTTGGACT3′			do	do	20	59
EG08R	5′TATCTGGTGGCGCAACTTTG3′					20	59