Fig 1. OPCs and oligodendrocytes derived from the monkey ESCs CMK6_SFF.

(A) Protocol for differentiation into OPCs and oligodendrocytes. (B–D) Immunostained CMK6_SFF before differentiation. Cultured cells in Step 0 immunostained with Nanog (green) and Hoechst (blue, B). Cells stained with Oct4 (red) and Hoechst (blue, C). Sample from panel C also stained with Sox2 (green, D). (E–G) Immunostained CMK6_SFF from Step 1. Cells from day 6 immunostained with Sox2 (green) and Hoechst (blue, E). Cells stained with Pax6 (green), Sox1 (red), and Hoechst (blue, F), as well as Nkx2.2 (green), Olig2 (red), and Hoechst (blue, G). (H–J) Immunostained CMK6_SFF from Step 2. Cells were cryopreserved for 581 days and the cells from day 59 immunostained with Nkx2,2 (green), Olig2 (red), and Hoechst (blue, H). Cells stained with PDGFRα (green) and Hoechst (blue, I), as well as with A2B5 (green), Olig2 (red), and Hoechst (blue, J). (K–M) Immunostained CMK6_SFF from Step 3. Cells from day 73 immunostained with O4 (green) and MBP (red). Scale bars = 50 μm.