TABLE 1:
Summary of Fcil/Fam ratios and overall expression levels.
| Construct | N(cells) | N(expt) | Mean Fcil/Fam | Mean Ftotal counts/μs (×106) | Range Ftotal counts/μs (×106) |
|---|---|---|---|---|---|
| SR3-GFP | 21 | 5 | 11.3 ± 1.27 | 0.36 ± 0.06 | 4.3 |
| SR3-GFP (no cilia) | 11 | 3 | – | 1.09 ± 0.18 | 2.0 |
| SR3-c8R-GFP | 10 | 3 | 2.07 ± 0.41 | 1.28 ± 0.19 | 2.7 |
| SR3-GFP-c8R | 36 | 6 | 1.87 ± 0.23 | 2.91 ± 0.41 | 10.7 |
| SR3-c8Rm-GFP | 12 | 3 | 5.87 ± 0.65 | 1.01 ± 0.08 | 1.2 |
| SR3-i3R-GFP | 21 | 5 | 3.37 ± 0.55 | 1.40 ± 0.27 | 3.7 |
| SR3-ΔSCTS1-GFP | 17 | 5 | 3.70 ± 0.55 | 0.89 ± 0.18 | 2.8 |
| SR3-ΔSCTS2-GFP | 14 | 4 | 3.01 ± 0.56 | 2.40 ± 0.36 | 4.5 |
| SR3-ΔSCTS1,2-GFP | 23 | 6 | 3.70 ± 0.27 | 0.50 ± 0.06 | 0.95 |
| Rho-GFP | 11 | 3 | 0.50 ± 0.04 | 3.05 ± 0.48 | 4.7 |
| Rho-GFP (low expression) | 16 | 3 | 0.27 ± 0.08 | 0.31 ± 0.29 | 0.67 |
| Rho-GFP-c8R | 23 | 5 | 1.08 ± 0.23 | 1.43 ± 0.48 | 10 |
| Rho-Δc8-GFP | 12 | 3 | 0.64 ± 0.14 | 0.82 ± 0.22 | 2.7 |
| Rho-i3S-GFP | 20 | 4 | 3.01 ± 0.46 | 3.49 ± 0.65 | 15 |
| Rho-i3S-GFP (low expression) | 21 | 3 | 2.75 ± 0.51 | 0.37 ± 0.10 | 1.6 |
| Rho-i3S-GFP-c8R | 17 | 4 | 4.48 ± 0.60 | 1.45 ± 0.40 | 7.3 |
| Rho-i3S-Δc8R-GFP | 8 | 3 | 7.61 ± 1.12 | 0.66 ± 0.09 | 0.98 |
| Rho-i3S-Δc4R-GFP | 13 | 3 | 8.54 ± 0.89 | 1.80 ± 0.50 | 5.7 |
N(cells) is the total number of cells analyzed. N(expt) is the number of discrete experiments (i.e., experiments that took place on different days with distinct cell cultures and ciliogenesis induction). Range refers to the highest value minus the lowest value in the data set. Low-expression experiments used lower concentrations of plasmid for transfection (20% of that used in all other experiments).