Skip to main content
. 2017 Feb 15;28(4):554–566. doi: 10.1091/mbc.E16-07-0549

TABLE 1:

Summary of Fcil/Fam ratios and overall expression levels.

Construct N(cells) N(expt) Mean Fcil/Fam Mean Ftotal counts/μs (×106) Range Ftotal counts/μs (×106)
SR3-GFP 21 5 11.3 ± 1.27 0.36 ± 0.06 4.3
SR3-GFP (no cilia) 11 3 1.09 ± 0.18 2.0
SR3-c8R-GFP 10 3 2.07 ± 0.41 1.28 ± 0.19 2.7
SR3-GFP-c8R 36 6 1.87 ± 0.23 2.91 ± 0.41 10.7
SR3-c8Rm-GFP 12 3 5.87 ± 0.65 1.01 ± 0.08 1.2
SR3-i3R-GFP 21 5 3.37 ± 0.55 1.40 ± 0.27 3.7
SR3-ΔSCTS1-GFP 17 5 3.70 ± 0.55 0.89 ± 0.18 2.8
SR3-ΔSCTS2-GFP 14 4 3.01 ± 0.56 2.40 ± 0.36 4.5
SR3-ΔSCTS1,2-GFP 23 6 3.70 ± 0.27 0.50 ± 0.06 0.95
Rho-GFP 11 3 0.50 ± 0.04 3.05 ± 0.48 4.7
Rho-GFP (low expression) 16 3 0.27 ± 0.08 0.31 ± 0.29 0.67
Rho-GFP-c8R 23 5 1.08 ± 0.23 1.43 ± 0.48 10
Rho-Δc8-GFP 12 3 0.64 ± 0.14 0.82 ± 0.22 2.7
Rho-i3S-GFP 20 4 3.01 ± 0.46 3.49 ± 0.65 15
Rho-i3S-GFP (low expression) 21 3 2.75 ± 0.51 0.37 ± 0.10 1.6
Rho-i3S-GFP-c8R 17 4 4.48 ± 0.60 1.45 ± 0.40 7.3
Rho-i3S-Δc8R-GFP 8 3 7.61 ± 1.12 0.66 ± 0.09 0.98
Rho-i3S-Δc4R-GFP 13 3 8.54 ± 0.89 1.80 ± 0.50 5.7

N(cells) is the total number of cells analyzed. N(expt) is the number of discrete experiments (i.e., experiments that took place on different days with distinct cell cultures and ciliogenesis induction). Range refers to the highest value minus the lowest value in the data set. Low-expression experiments used lower concentrations of plasmid for transfection (20% of that used in all other experiments).