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. 2017 Feb 13;12(2):e0171435. doi: 10.1371/journal.pone.0171435

Fig 1. RNA-seq cell line authentication pipeline.

Fig 1

Raw RNA-seq data is aligned to the human genome using STAR, followed by processing and variant calling steps using GATK tools. Non-SNV variants (insertions, deletions, etc.) are removed, and the resulting SNVs are annotated using SnpEff and SnpSift. SNVs passing the GATK variant filtration step and having a total allelic depth of at least 10 are compared to COSMIC SNV profiles filtered to include unique SNV positions, as well as a SNV genotyping panel.