Figure 3.

[A] Table showing results from thrombin generation assay indicates that SynthoPlate^TM itself does not have the capability to rapidly activate coagulation factors in plasma to generate thrombin, since just adding SynthoPlate^TM to re-calcified PRP or PPP without Tissue Factor (TF) or platelet agonist (e.g. ADP) has no accelerating effect on thrombin generation, and the thrombin generation is enhanced only upon TF and/or ADP addition to PRP or PPP, with SynthoPlate^TM slightly enhancing this effect (possibly by recruitment and clustering of active platelets in suspension); [B] Representative fluorescence images at 15 min time-point, as well as, [C] surface-averaged fluorescence intensity values at 5 min and 15 min time-points of fibrin(ogen) generation/deposition on ‘vWF + collagen’ surface in the PPFC experiments show that, resting platelets (w/o ADP) in 50% PRP (diluted by PPP) generate/deposit only minimal levels of fibrin(ogen) as they interact with the ‘vWF + collagen’ surface and this generation/deposition increases if platelet agonist is introduced in the flow (i.e. w ADP); introducing SynthoPlateTM vesicles in the flow along with ADP-activated platelets in 50% PRP significantly enhances the fibrin(ogen) generation/deposition during the 15 min flow period, compared to flow with ‘adhesion only’ particles (control) and even compared to flow of 100% PRP (w ADP) without SynthoPlate^TM; [D] D-dimer analysis of the generated/deposited clots on the ‘vWF + collagen’ surface confirms that the enhanced clot formation in the SynthoPlate^TM-added group is indeed rich in fibrin and not just fibrinogen binding to recruited active platelets.