Figure 5.

Representative immunofluorescence microscopy images of tail tissue cryo-sections (transverse section) proximal to hemostasized injury site showing vascular endothelium (blue CD31), particles (red RhB), platelets (green CD41), and corresponding overlays, indicate that [A] tail tissues from SynthoPlate^TM-injected mice have significant co-localization of endothelium, particles and platelet fluorescence, while [B] that from control particle-injected mice show minimal co-localization, representative scale bar 25μ; [C] percent (%) of blood vessels showing significant co-localization of particle fluorescence (red) and platelet fluorescence (green) was significantly higher in SynthoPlate^TM-injected TCP mice versus control (unmodified) particle-injected mice; (‘***’ indicates p ≤ 0.001); [D] immunoblot analysis of injured tail tissue from SynthoPlate^TM-treated TCP mice shows a significantly higher presence of high molecular weight fibrin, compared to that from control particle-treated TCP mice.