Table 2.

Characteristics of the eight microsatellite loci used in the study

Locus	Sequence 5′–3′	PCR	Repeat	A	Size bp	H o	H s	H t
EP1	F: TGG TGC CTC ATG TGT TCC G	57°	ACAT	18	146–218	0.686	0.665	0.838
	R: GTG GCG GAT GGA AGC AAA G
EP7	F: TCC AGG TTG GCT TGG ATG G	57°	ATGT	11	244–285	0.389	0.428	0.705
	R: AGA ACA GGC CAC AAC AGA G
EP8	F: TCT TTC TAA GAT TAT GGA ACG CC	57°	TGAA	9	168–204	0.44	0.536	0.692
	R: TTC GCA GTC CCA GAT AGC C
EP16	F: GCT ATG TAT CTG TGC GTG C	57°	AAAT	10	164–208	0.369	0.405	0.438
	R: GGT TTC ACT GCC CGC TTA C
EP21	F: GCA CTC CAC TGC CTT TTG G	Multi 58°	ACAT	12	164–215	0.574	0.785	0.87
	R: TAT GAA TTG CCC AGT GCC
EP29	F: ACG AAT CGG CAG TGT CCA G	59° Mg 3.0	AAT	14	180–231	0.402	0.513	0.758
	R: AGC TTG TGT CAG TCT GGT TTG
EP31	F: ACT GGA AAA AGC TAA TTG ATA GAT G	57°	AGAT	31	159–299	0.596	0.859	0.924
	R: TTG CTT ATG TAA ATT GGA AAT GTT
EP33	F: GTG CAC CCA GTC AAT CAC AC	59° Mg 2.5	AATC	12	187–223	0.662	0.726	0.778
	R: ACT GAA TCG CAG ACC CAG

PCR, primer‐specific PCR conditions including annealing temperature; multi, Qiagen Multiplex mastermix (all others in Qiagen HotStarTaqPlus mastermix); Mg, final concentration of MgCl₂ (mmol/L) if supplemented. F, forward; R, reverse.A, total number of alleles in 168 individuals from 13 sampling locations; size: range of allele sizes; bp, base pairs. Nei's diversity statistics (Nei, 1973) were calculated in FSTAT jackknifed across populations, using nine populations comprising 14–32 individuals, H _o, observed heterozygosity; H _s, expected heterozygosity under HWE within subpopulations; H _t, expected heterozygosity in total population.