Table 7.
Target genea | Tu‐YN | Tc‐YN | ||||
---|---|---|---|---|---|---|
Nucleotideb | Amino acidc | Matchd | Nucleotide | Amino acid | Match | |
VGSC | 11 | 1 | – | 10 | 1 | – |
GluCl‐01 | 2 | 1 | – | 1 | 0 | – |
GluCl‐02 | 1 | 1 | – | 1 | 1 | – |
GluCl‐03 | 1 | 1 | – | 0 | 0 | – |
GluCl‐04 | 2 | 0 | – | 3 | 1 | – |
GluCl‐05 | 0 | 0 | – | 1 | 0 | – |
Rdl1 | 3 | 2 | – | 2 | 1 | – |
Rdl2 | 4 | 0 | – | 3 | 1 | – |
Rdl3 | 1 | 0 | – | 1 | 0 | – |
SdhA | 6 | 2 | – | 5 | 1 | – |
SdhB | 1 | 0 | – | 2 | 0 | – |
SdhC | 4 | 1 | – | 2 | 0 | – |
SdhD | 0 | 0 | – | 1 | 0 | – |
SdhE | 0 | 0 | – | 0 | 0 | – |
Total | 36 | 9 | – | 32 | 6 | – |
VGSC: voltage‐gated sodium channel gene; GluCl‐01~GluCl‐05: 5 glutamate‐gated chloride channel genes; Rdl1–Rdl3: 3 GABA receptor genes; SdhA–SdhE: 5 succinate dehydrogenase complex genes.
Alignment between tested cDNA sequence and baseline at the level of nucleotide.
Alignment between tested cDNA sequence and baseline at the level of amino acid.
Identified point mutation from amino acid sequence was matched with the reported mutations of target genes involved in acaricide resistance in mites; “–” indicates not match.