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. 2017 Feb 14;8:112. doi: 10.3389/fimmu.2017.00112

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Copyright © 2017 Lin, Yihao, Zhou, Yin, Qiang, Haowen, Qianwei, Jun, Yuan, Gang, Hua, Yunfeng and Zhi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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M1/M2-associated markers of microglia at protein level. Perihematomal tissue was costained for CD68 (M1 marker) (green) and Iba1 (microglia marker) (red), or CD206 (M2 marker) (green) and Iba1 (red) at 24 h after intracerebral hemorrhage (ICH) (A). Cell nucleus was stained with DAPI (blue). White arrows pointed to typical cells. n = 6 in per group. CD68 (B) and CD206 (C) were also tested using western blots. Values of the relative densitometric analysis were expressed as mean ± SD, n = 6 in each group. **P < 0.01 compared with sham group; ^##P < 0.01 compared with ICH + Vehi group; ^&P < 0.05 compared with ICH + JWH group.