Figure 7.

Immunohistochemistry evaluating the number of (A) pyknotic nuclei in the CA3 of the hippocampus at 15 days post-injury, showing an increased number of pyknotic cells in brain-injured animals compared to sham- and naïve controls (*p < 0.05, Figure 8). AF-16-treated animals had similar cell counts as scramble-treated controls, regardless of injury status (Figure 8). (B–D) Representative examples from a naïve animals (B) and brain-injured animals (C,D) subjected to midline fluid percussion injury (mFPI) and treated with inactive control, scramble, compound (C), or the active compound AF-16 (D).