Fig. 3.

A local accumulation of actin filaments precedes plug formation and is associated with the expanding plug during plug deposition. Plug formation over time (a–g) visualized by maximum intensity projections of Z-stacks of a hypha of a Lifeact-eGFP expressing P. infestans strain with the hyphal tip (not visible) pointing upwards. The arrowhead in a indicates the plug deposition site. The insets in a–g are orthogonal views of the Z-stacks at the site where the plug is deposited. h is a bright field image of d. i Kymograph of the plug deposition. j Average fluorescence intensity in percentage (Y-axis) over time of the plaques that remain in the basal part of the hypha after plug formation (n = 3, dark gray line) with the average intensity over time of randomly picked plaques in the apical part of the hypha (n = 7, light gray line) set to 100 %. At t = 8 (first arrow) plug deposition is first visible and at t = 20 plug formation is completed (second arrow). Error bars indicate standard deviations. Bar 5 μm