Fig. 6.

Isolation of C41(DE3) from BL21(DE3). To isolate C41(DE3), BL21(DE3) was first transformed with a T7-based expression vector harbouring the gene encoding the mitochondrial oxoglutarate malate carrier protein (OGCP) and expression of ogcp was induced with IPTG in liquid culture. Notably, the ogcp expression vector has an ampicillin resistance marker. Surviving cells were selected for on IPTG-containing agar plates and subsequently probed for efficient OGCP production. In a second step, selected clones were cured from the ogcp expression vector by culturing them for a prolonged period of time in a closed setup

(modified after [143])