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Oxidized RNAs accumulate during aging and in yeast decapping mutants. Total RNA was prepared from wt (CML39-11A) and Kllsm4Δ1 cells after 1 day (lane 1–2) and 3 days (lane 3–4) of growth. RNAs were separated by gel electrophoresis and transferred to Opitran filter. a The presence of oxidized RNAs was revealed by the 15A3 antibody, which recognizes 8-oxo-7,8-dihydro- guanosine (8OHG). b Ethidium bromide staining was used to visualize ribosomal RNAs
