Fig. 1.
Co-localization of the GR with members of the Bcl-2 protein family: Bak, Bax, Bcl-xL and Bim in DP thymocytes. Representative confocal microscopic images from at least three independent experiments showing GR-Bak (A1), GR-Bax (B1), GR-Bcl-xL (C1) and GR-Bim (D1) co-localization in control (Ctrl) and 30 min DX-treated cells. DIC, CD4 (blue channel) and CD8 (virtual red channel) overlaid, intracellular GR (green channel) and Bak, Bax, Bcl-xL, Bim (red channel) images are shown. The co-localization of the GR with Bak, Bax, Bcl-xL and Bim (GR-Bak, Bax, Bcl-xL, Bim merged images) is indicated by the yellow areas. Scale bars 8 µm each. Bar diagrams show the quantification of the changes in the GR-Bak (A2), GR-Bax (B2), GR-Bcl-xL (C2) and GR-Bim (D2) co-localization in DP thymocytes after in vitro DX treatment. Bars represent the number of co-localized pixels per cell as calculated by the co-localization plugin of the ImageJ software. The mean ± SEM was calculated from the data of 100 DP cells per treatment, respectively. Significant changes (p < 0.05) in DX-treated cells versus controls are indicated by asterisk. (Color figure online)