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. 2017 Feb 14;8:115. doi: 10.3389/fimmu.2017.00115

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Copyright © 2017 Minigo, Flanagan, Slattery and Plebanski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunization with variant DI but not variant AI inhibits KI-specific IFNγ-secreting effector T cells in vivo. BALB/c mice were immunized with dendritic cells (DCs) pulsed with KI on day 0 (KI), DCs pulsed with DI on day 14 (DI) or with KI-pulsed DCs on day 0 followed by DI-pulsed DCs on day 14 (KI–DI). The negative control group received two immunizations with non-pulsed DCs 14 days apart. Splenocytes were assessed for KI-specific (black bars) and DI-specific (gray bars) (A) IFNγ, (B) IL-4, and (C) IL-10 responses by ex vivo ELISpot assay 17 days after the last immunization (day 31). (D) BALB/c mice were immunized with DCs pulsed with KI on day 0 (KI), with DCs pulsed with AI on day 14 (AI) or with KI-pulsed DCs on day 0 followed by AI-pulsed DCs on day 14 (KI–AI). The negative control group received two immunizations with non-pulsed DCs 14 days apart. Splenocytes were assessed for KI-specific (black bars) and AI-specific (gray bars) IFNγ responses by ex vivo ELISpot assay 14 days after boost immunization (day 28). Mean spot forming units ± SD (n = 3 mice per group). Two-way ANOVA with Tukey’s multiple comparison test was used to test for statistical significance when comparing all groups (A,B,D). Two-way ANOVA with Dunnett’s multiple comparison test was used to test for statistical significance when comparing to the media control (C).