Figure 6.

KI/AI-primed cells are resistant to DI-induced immune interference. BALB/c mice were immunized on day 0 with dendritic cells (DCs) pulsed with either KI or AI or with DCs pulsed with KI for 1 h followed by AI for another 2 h. On day 14, three groups received sequential immunization with DI-pulsed DCs (KI–DI, KI/AI–DI, and AI–DI). The negative control group received two immunizations with non-pulsed DCs 14 days apart. Splenocytes were assessed for KI-specific IFNγ responses by ex vivo ELISpot assay 17 days after boost immunization (day 31). Mean spot forming units ± SD are shown (n = 3 mice per group). Two-way ANOVA with Dunnett’s multiple comparison test was used to test for statistical significance when compared to the groups immunized with the index peptide KI.