Table 1.

Vitrification solutions for ovarian tissue preservation

Vitrification solution components					Analyses (ovary)	Citations
EG	DMSO	G	S	Polymer
X	X				In vitro culture, histology, apoptosis, and secreted E2 and P4 in human	[15]
X	X		X		Autotransplant, serum E2, growth of primordial follicles in bovine; morphology in human	[16]
					Xenograft and recovery of primordial or secondary follicle-containing bovine tissue into mice	[17]
X	X			PrOH, PVP	Histology and ultrastructure in human	[11, 12]
X			X	PVP	Histology, follicle count, and ultrastructure in cynomolgus macaques	[18]
					Autotransplant, histology and ultrastructure, vascularization of graft, serum E2 and P4, ova stimulation, and embryo development through ICSI in cynomolgus macaques	[19]
					Autotransplant, serum E2 and P4, ova stimulation, and embryo development through ICSI, 2 successful deliveries in human	[20]
X	X		X	PVP K-10	Apoptosis, proliferation, and fibrosis of human xenografted in mice	[21]
					Autotransplant, histology, follicle count, proliferation, apoptosis, graft vascularization and fibrosis, AMH immunohistochemistry in Papio anubis	[22]
					Autotransplant, histology, follicle count, proliferation, apoptosis, graft vascularization and fibrosis, AMH immunohistochemistry in cynomolgus macaques	[23]
X		X		PVP K-12, X-1000, Z-1000	Histology, proliferation, follicle counts, in vitro growth and hormone secretion in rhesus macaque	[7]
					In vitro growth and hormone secretion of follicles isolated from vitrified rhesus macaque tissue	[8]

Examples of vitrification solutions that have been used for human, bovine, and non-human primate ovarian tissue preservation. A brief description of their analysis is listed.

E2 estradiol, P4 progesterone, DMSO dimethyl sulfoxide, EG ethylene glycol, G glycerol, PrOH 1,2-propanediol (a.k.a. propylene glycol), PVP polyvinylpyrrolidone, S sucrose, X-1000 and Z-1000 21st Century Medicine Super cool polymers