Figure 7.

Determination of mitochondrial membrane potential (ΔΨm). Rhodamine 123 (RH-123), a mitochondria specific fluorescent dye which stains live cells, is used to determine the mitochondrial membrane potential by measuring fluorescence intensity under microscope. MCF 7 cells were treated with different concentrations of AEEA for 24 h and stained with Rhodamine 123 following standard protocol and observed under fluorescence microscopy. (a) Control (without treatment); (b–d) treatment with various concentrations of Eclipta alba extract. ∗ represents p ≤ 0.05.