Figure 5.

p-p38 MAPK 45-50 min after onset of different stimulus protocols. A, Representative confocal images of p-p38 MAPK immunofluorescence in SNs at 45-50 min after onset of 5-HT. B, Summary data. Levels of p-p38 MAPK was measured at 45 min after onset of a single 25-min 5-HT pulse, two 5-min duration pulses of 5-HT with ISI of 20 min, or one 5-min duration pulse of 5-HT. p-p38 MAPK was measured at 50 min after onset of two pulses of 5-HT with an ISI of 45 min. The last group produced less p-p38 MAPK than any of three former groups. Scale bar, 20 μm. Significant differences are indicated by * for p < 0.05.