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. 2017 Feb 14;7:42041. doi: 10.1038/srep42041

Figure 6. Suppression of IL-1β pro-inflammatory pathway attenuates the NG2 glial cell ablation-induced hippocampal cell death.

Figure 6

(a) Timeline of the experimental design for the treatment with rat recombinant IL-1 receptor antagonist (rrIL-1ra). (b) Bright-field immunohistochemical observations of NeuN in the hippocampal CA1 region of NG2-HSVtk transgenic rats treated with vehicle (Control), GCV for 2 days (GCV2d), or co-administration of GCV and IL-1ra (1 μg/day) for 2 days following IL-1ra administration for 5 days (GCV2d + IL-1ra). (c) Relative expression levels of TNFα mRNAs in the hippocampi of NG2-HSVtk transgenic rats treated with vehicle (Control, value set as 1.0), GCV for 1 day, or co-administration of GCV and IL-1ra for 1 day following IL-1ra administration for 5 days. (d) Confocal images depicting microglia (Iba1, green) in the hippocampus CA1 region of NG2-HSVtk transgenic rats treated with vehicle, GCV for 2 days, or co-administration of GCV and IL-1ra for 2 days following IL-1ra administration for 5 days. Hoechst, cell nuclear staining. Mean ± SD, n = 2 rats (Control) and 3 rats (GCV1d, GCV2d, GCV1d + IL-1ra, GCV2d + IL-1ra); *p < 0.05, based on a Student’s t-test analysis. Scale bars represent 100 μm (b) and 20 μm (d).