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. 2017 Feb 14;7:42498. doi: 10.1038/srep42498

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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. (a) The predicted binding sequence of let-7e in lnc-MKI67IP-3 sequence. The solid line represents standard base pairing and strong interactions; and the dashed line represents non-standard base pairing and relatively weak interactions. (b) Upper panel: the sequence alignment between let-7e and mutant of let-7e binding sequence in lnc-MKI67IP-3. The bold letters indicated the mutated sequence. Lower panel: HUVECs were transfected alone or co-transfected with different combinations as shown in the figure for 24 hours. Then, the luciferase activity in each group was assayed. HUVECs transfected with non-insert reporter (empty) vector were used as control. *p < 0.05 vs. all other groups. All experiments were repeated five times independently.