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. 2017 Feb 14;7:42476. doi: 10.1038/srep42476

Figure 1. The nonagouti (a) mutation in MSM/Ms mice.

Figure 1

(a) Schematic representation of sgRNA targeting nonagouti (a). (b) Founder (F0) knockout (KO) and wild-type (WT) mice. The F0 mice had black coats, indicating loss of the agouti protein. (c) DNA sequencing analysis of the CRISPR target site in the MSM/MS WT and founder mice. Indels (insertions, blue bold characters; deletions, dashed lines) were identified at the sgRNA targeted loci. Black and red bold characters represent sgRNA recognition sites and PAM sequences, respectively. Four mutated alleles (A to D) were observed in the founder KO mice. (d) DNA sequencing analysis of homozygous KO mice (F2) from lines A (upper) and D (lower).