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. 2017 Feb 14;7:42476. doi: 10.1038/srep42476

Table 1. Generation of nonagouti-knockout MSM mice by the CRISPR/Cas9 system.

Target (CRISPR construct) Injected Cultured (%) 2-cells (%) Transferred Pups born (%) Pups weaned (%) Homozygous knockout (% per pups)
#1 54 38 (70) 35 (65) 35 0 0 0
#2 39 29 (74) 29 (74) 29 2 (7) 2 (7) 2 (100)
#3 34 28 (82) 28 (82) 28 1 (4) 1 (4) 1 (100)
Total 127 95 (75) 92 (72) 92 3 (3)* 3 (3) 3 (100)

*All male pups.