Table 1. Generation of nonagouti-knockout MSM mice by the CRISPR/Cas9 system.
Target (CRISPR construct) | Injected | Cultured (%) | 2-cells (%) | Transferred | Pups born (%) | Pups weaned (%) | Homozygous knockout (% per pups) |
---|---|---|---|---|---|---|---|
#1 | 54 | 38 (70) | 35 (65) | 35 | 0 | 0 | 0 |
#2 | 39 | 29 (74) | 29 (74) | 29 | 2 (7) | 2 (7) | 2 (100) |
#3 | 34 | 28 (82) | 28 (82) | 28 | 1 (4) | 1 (4) | 1 (100) |
Total | 127 | 95 (75) | 92 (72) | 92 | 3 (3)* | 3 (3) | 3 (100) |
*All male pups.