Figure 1. dSTIM is required in the nervous system during early pupal development for flight.

(a) Schematic of the assay used for measuring duration of flight bouts. (b) Duration of flight bouts in flies with pan-neuronal knockdown of dStim at different developmental stages is plotted as box plots. Stages of dStim knockdown are indicated below each box. Stage specific knockdowns were performed with the TARGET system, where a GAL80^ts transgene was driven by the tubulin promoter (tubGAL80^ts). Horizontal lines in the box represent medians, crosses indicate the means, box limits indicate the 25th and 75th percentiles, whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, individual data points are represented as open circles and the numbers below represent the number of flies tested for each box. (c) Bars represent dSTIM protein levels (means ± SEM) normalized to Tubulin in protein lysates isolated from CNS of the indicated genotypes. A representative western blot used for quantification is shown below. n ≥ 3. Red alphabets over the box plots/bar graphs represent statistically indistinguishable groups (one-way ANOVA with a post hoc Tukey’s test p < 0.05). APF- After Puparium Formation.