Figure 5. Over-expression of Ral can rescue flight deficits caused by loss of intracellular calcium signalling.

(a,b) Fold change of Ral RNA levels obtained by qPCR from the indicated tissues, genotypes and ages represented as bars with error bars indicating SEM. (c,d) Box plots show the flight bout durations of female flies of the indicated genotypes. Over-expression of Ral^WT can partially rescue flight upon knockdown of the SOCE components dStim and dOrai. (e) Reduced flight bout durations in IP₃R knockdown (itpr^IR) and (f) mutant (itpr^wc361/ka1091) strains can be rescued by over-expression of Ral^WT. Box plots and their symbols are as described for Fig. 1b. Red alphabets over the box plots represent statistically indistinguishable groups (one-way ANOVA with a post hoc Tukey’s test p < 0.05). *p = 0.096, **p = 0.0073, ***p = 0.001; two-tailed t-test.