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. 2017 Jan 23;114(6):E990–E998. doi: 10.1073/pnas.1615758114

Fig. S2.

Fig. S2.

p53 regulation under hypoxia. (A) HeLa and SiHa cells were cultivated under normoxia (21% O2) or hypoxia (1% O2). Shown are the mean p53 transcript levels measured by qRT-PCR after 24 h under hypoxia for each cell line relative to the corresponding p53 transcript levels under normoxia (set at 1.0). SDs of biological replicates are indicated (n = 4), n.s., statistically not significant. (B) HPV18-positive HeLa, HPV16-positive SiHa, and HPV-negative HCT116 cells were cultivated for 24 h under normoxia (21% O2) or hypoxia (1% O2), in either the absence (−) or the presence (+) of 10 μM Nutlin-3. Shown are immunoblots of p53 and HPV16/18 E7 protein levels. β-actin served as a loading control. n.d., not determined.