Multiplex immunofluorescence analysis of an HPV16-positive cervical cancer specimen. (Upper, Left) Expression of CD34 (red) and E7 (orange). (Upper, Right) Expression of CD34 (red) and CA IX (green). (Lower, Left) Expression of CD34 (red), E7 (orange), and CA IX (green). (Lower, Right) Expression of CD34 (red), CA IX (green), and Ki-67 (white). Dotted lines surround CA IX-positive/E7-negative regions in which the expression of Ki-67 is strongly reduced or absent. (Scale bar: 100 µm.)