Quantitative real-time primers |
β-actin |
F: GATTACTGCTCTGGCTCCTAGC
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R: GACTCATCGTACTCCTGCTTGC
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IDO |
F: AAGAAGTGGGCTTTGCTCTG
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R: CGCTGTGACTTGTGGTCTGT
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hGBP1 |
F: GTGGAACGTGTGAAAGCTGA
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R: CAACTGGACCCTGTCGTTCT
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hGBP2 |
F: GATTTCACCCTGGAACTGGA
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R: GGGTTCAGCTCTTCCTCCTT
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hGBP3 |
F: TTAATCTGCCCCGACTCTGT
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R: CATTGACCTTGATGCCTCCT
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hGBP4 |
F: GGCCCAAATGGAGAAGAAGT
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R: AGCCCCAGGTAGAGTGACAA
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hGBP5 |
F: AGGCCAAAGCAAGGTAGTGA
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R: ATGATGCCACCTGGAAGAGT
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iNOS |
F:TTTCCAAGACACACTTCACCA
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R: TCCTTTGTTACCGCTTCCAC
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siRNA |
siIDO1 |
Sense: TGTTACAATGGGTAATGACAG
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Antisense: GTCATTACCCATTGTAACAGA
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siIDO2 |
Sense: TATAATTACACATAATTACCT
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Antisense: GTAATTATGTGTAATTATACT
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siIDO3 |
Sense: TTAACTTCTCAACTCTTTCTC
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Antisense: GAAAGAGTTGAGAAGTTAAAC
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Nontargeting control (siCtrl) |
Sense: AACAAGATGAAGAGCACCAA
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Antisense: TTGGTGCTCTTCATCTTGTT
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Short hairpin RNA primers |
shGBP1 |
AATTCAAAAACCAGATGAGTACCTGACATACCTCGAGGTATGTCAGGTACTCATCTGG |
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CCGGCCAGATGAGTACCTGACATACCTCGAGGTATGTCAGGTACTCATCTGGTTTTTG |
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shGBP2 |
AATTCAAAAAGCAATTCAACTCATGCTTATTCTCGAGAATAAGCATGAGTTGAATTGC |
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CCGGGCAATTCAACTCATGCTTATTCTCGAGAATAAGCATGAGTTGAATTGCTTTTTG |
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shGBP5 |
AATTCAAAAACAAGGTAGTGATCAAAGAGTTCTCGAGAACTCTTTGATCACTACCTTG |
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CCGGCAAGGTAGTGATCAAAGAGTTCTCGAGAACTCTTTGATCACTACCTTGTTTTTG |