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. 2017 Jan 23;114(6):1424–1429. doi: 10.1073/pnas.1613069114

Fig. 3.

Fig. 3.

STV1 associates with pri-miRNAs but not the DCL1 complex. (A) STV1 does not interact with DCL1, HYL1, and SE. MYC-STV1 was coexpressed with DCL1-YFP, SE-YFP, or HYL1-YFP in N. benthamiana. After protein extraction, MYC-STV1 was immunoprecipitated with anti-MYC antibodies. MYC-STV1 and YFP-tagged proteins were detected with anti-MYC and anti-YFP antibodies, respectively, and indicated on the left. The interaction of SE and CDC5 is shown as a positive control. Numbers on top of the pictures indicate protein extracts containing: 1, YFP and MYC-STV1; 2, DCL1-YFP and MYC-STV1; 3, YFP and MYC-STV1; 4, SE-YFP and MYC-STV1; 5, YFP and MYC-STV1; 6, HYL1-YFP and MYC-STV1; 7, YFP and MYC-SE (Middle); 8, YFP-CDC5 and MYC-SE (Top). (B) STV1 binds pri-miRNAs in vivo detected by RIP. Inflorescences from WS and stv1-1 harboring MYC-STV1 were used for RIP assay with anti-MYC antibodies. No antibody (No Ab.) was used as a negative control. Ten-percent of IPs and 2% input proteins were used for Western blot. Five percent RNAs were used as input RNA. STV1 on the top of the picture indicates MYC-STV1.