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. 2017 Jan 23;114(6):1424–1429. doi: 10.1073/pnas.1613069114

Fig. S4.

Fig. S4.

The function of STV1 in the miRNA pathway. (A) The transcript levels of several genes required for miRNA biogenesis detected by qRT-PCR. The transcript levels of examined genes were normalized to those of UBQ5 and compared with WS (value set as 1). Means of three replicates were shown. **P < 0.01. (B) Protein levels of DCL1, HYL1, and SE in WS and stv1-1 detected by Western blot. The large subunit of Rubisco (RbcL) was stained as a loading control. (C) STV1 does not interact with CDC5. 1, protein extracts containingYFP-CDC5 and MYC-STV1; 2, protein extracts containing YPF-CDC5 and MYC-DCL1. The DCL1-CDC5 interaction is shown as a positive control. (D) STV1 does not interact with DDL. MYC-STV1 was coexpressed with YFP-CDC5 or YFP-DDL in N. benthamiana. 1, protein extracts containing YFP-DDL and MYC-STV1; 2, protein extracts containing YPF-DDL and MYC-DCL1. MYC-STV1 was IPed with anti-MYC antibodies. MYC-STV1 and YFP-tagged proteins were detected with anti-MYC and anti-YFP antibodies, respectively, and indicated on the left. The DCL1–DDL interaction is shown as a positive control.