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Functional analysis of distal site mutants. (A) Kinetics of Ub charging and discharging for the indicated WT (wt) and mutant IpaH9.8 NEL domains. (B) Binding analysis of UBCH5 to the indicated WT or mutant IpaH9.8 NEL domains using MST. (Inset) Calculated Kd values (n = 3).
