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IpaH autoinhibition involves a short-circuit mechanism. (A) Kinetics of Ub charging and discharging for WT (wt) or discharge mutant (D339R) full-length (FL) autoinhibited IpaH9.8. (B) Kinetics of Ub charging and discharging for WT FL and isolated NEL domain of IpaH9.8. (C) Kinetics of Ub charging and discharging for catalytic base (D552) mutants of FL and isolated NEL domain of SspH1 in the presence or absence of the substrate, PKN1 fragment HR1b (HR1b).
