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. 2017 Jan 23;114(6):1311–1316. doi: 10.1073/pnas.1611595114

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Fig. S4. — Comparison of full-length (FL) versus isolated NEL domain in Ub charging and discharging reactions. (A) Quantification of Ub-charged intermediates over time for reactions involving the FL or isolated NEL domain of IpaH9.8 shown in Fig. 4B. (B) In vitro Ub charging and discharging kinetics for WT SspH1 FL and a catalytic cysteine mutant (C492A). (C) Kinetics of Ub charging and discharging for WT SspH1 FL and isolated NEL domain in conditions favoring visualization of the thioester intermediate. (D) Ub discharging kinetics for C337S mutants of the IpaH9.8 FL or isolated NEL domain. Quantification of Ub-charged intermediates is shown at the right.