Table S1.
Ecdysone-related genes with significant differential expression between winged offspring producing (induced) and wingless offspring producing (uninduced) pea aphid mothers
| Gene | Putative identity | Relation to ecdysone | P value* | Fold change & direction† |
| ACYPI085492 | Eclosion hormone (EH, paralog 2) | Activated by dropping ecdysone levels (14) | 0.0001 | 2.9× up in induced |
| ACYPI062598 | Crustacean cardioactive peptide (CCAP) | Activated by dropping ecdysone levels (14) | 0.0003 | 2.2× up in induced |
| ACYPI42083 | Eclosion hormone (EH, paralog 1) | Activated by dropping ecdysone levels (14) | 0.0004 | 2.6× up in induced |
| ACYPI005281 | Bursicon | Activated by dropping ecdysone levels (14) | 0.01 | 1.9× up in induced |
| ACYPI007510 | Prothoracicostatic peptide (PTSP) | Inhibits ecdysone release (13) | 0.01 | 1.9× up in induced |
| ACYPI001692 | Ecdysone receptor (EcR) | Ecdysone receptor (15) | 0.0001 | 3.3× up in uninduced |
| ACYPI005934 | Ultraspiracle protein (Usp) | 2.2× up in uninduced | ||
| ACYPI004047 | Chromatin-remodeling complex atpase chain ISWI (paralog 2) | Coactivator of EcR (53) | 0.001 | 2.5× up in uninduced |
| ACYPI40836 | Broad-complex core protein isoform | Ecdysone-inducible, primary response gene (16) | 0.009 | 3.0× up in uninduced |
| ACYPI083237 | Nuclear hormone receptor ftz-f1 | Coactivator of EcR (17) | 0.01 | 3.4× up in uninduced |
| ACYPI001312 | Chromatin-remodeling complex atpase chain ISWI (paralog 1) | Coactivator of EcR (53) | 0.046 | 3.7× up in uninduced |
| ACYPI25933 | E74a | Ecdysone-inducible, primary response gene (54) | 0.0001 | 2.1× up in uninduced |
| ACYPI49157 | DARK (apoptotic protease-activating factor 1) | Ecdysone inducible (55) | 0.0003 | 2.6× up in uninduced |
| ACYPI003094 | DRICE (Caspase-1) | Ecdysone inducible (55) | 0.048 | 1.7× up in uninduced |
| ACYPI007773 | E75 | Ecdysone-inducible, primary response gene (56) | ns | |
| ACYPI002959 | E93 | Ecdysone-inducible, primary response gene (57) | ns | |
| ACYPI37989 | Prothoracicotropic hormone (PTTH) | Activates ecdysone release (58) | ns | |
| ACYPI001519 | Spook (Spo) | Ecdysone biosynthesis (59) | ns | |
| ACYPI000716 | Spookiest (Spot) | Ecdysone biosynthesis (59) | ns | |
| ACYPI006623 | Phantom (Phm) | Ecdysone biosynthesis (59) | ns | |
| ACYPI006729 | Disembodied (Dib) | Ecdysone biosynthesis (59) | ns | |
| ACYPI000973 | Shadow (Sad) | Ecdysone biosynthesis (59) | ns | |
| ACYPI008228 | Shade (Shd) | Ecdysone biosynthesis (59) | ns |
*
P value from RNA-Seq experiment (12), false discovery rate corrected.
†
Bold genes exhibited higher expression in aphids induced to produce winged offspring, underlined genes had higher expression in uninduced aphids, and the remaining genes were not significantly differentially expressed.