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. 2017 Jan 23;114(6):1353–1358. doi: 10.1073/pnas.1614102114

Fig. S4.

Fig. S4.

Expression of dLdh correlates with L-2HG synthesis. RNAi was used to deplete dLdh mRNA transcripts in midsecond-instar larvae. Compared with the da-GAL4 and UAS-dLdhi control strains, da-GAL4 UAS-dLdhi larvae possess significantly lower levels of dLdh mRNA (A) and accumulate significantly less lactate and 2HG (B). In A, quantitative RT-PCR was used to measure the relative amount of dLdh mRNA. For all samples, transcript levels were normalized to the abundance of rp49 mRNA (n = 3 samples collected from independent populations with at least 10 midsecond-instar larvae per sample). In B, GC-MS data are represented and normalized as described in Fig. 1 (n = 6 samples collected from independent populations with 25 midsecond-instar larvae per sample). *P < 0.05; **P < 0.01.