Table S2.

M. marinum strains used in this paper

No.	Strain	Description	Antibiotic resistance	Fig.	Source
1	M strain	WT	None	Figs. 2 and 3	(62)
2	ΔRD1	M strain with a deleted RD1 locus.	None	Figs. 2 and 3	(29)
3	ΔRD1::2F9-EsxA-WT (aka ΔRD1::WTmt)	Strain 2 containing plasmid 3. M strain harboring a deleted RD1 locus heterologously expressing the Mtb RD1 locus on an integrating plasmid.	Hygromycin	Fig. 2	This work
4	WT::pYub412+tdtomato	M strain containing the empty vector pYUB412 and the fluorescent protein expressing psmp12::tdtomato vector. Contains plasmids 1 and 2.	Hygromycin, kanamycin	Figs. 1, 2, and 5	This work
5	ΔRD1::pYub412+tdtomato	ΔRD1 strain containing the empty vector pYUB412 and the fluorescent protein expressing psmp12::tdtomato vector. Contains plasmids 1 and 2.	Hygromycin, kanamycin	Figs. 1, 2, and 5	This work
6	ΔRD1::WTmt+tdtomato (ΔRD1::WTmt)	ΔRD1 strain containing the vector pYUB412 harboring the RD1 region of M. tuberculosis and the fluorescent protein expressing psmp12::tdtomato vector. Contains plasmids 1 and 3.	Hygromycin, kanamycin	Figs. 1 and 2	This work
7	ΔRD1:: 2F9-ESAT-6M93T+tdtomato (ΔRD1::M93Tmt)	Same as strain 5, except that it harbors plasmids 1 and 4, expressing EsxA-M93T.	Hygromycin, kanamycin	Fig. 1	This work