Fig. S2.

Generation of cell-type–specific WAVE1 KO mice. (A) WAVE1 D1-KO or D2-KO mice were generated by breeding floxed WAVE1 mice with Drd1a– or Drd2–Cre-recombinase mice. Exon 4 of the WAVE1 gene was flanked by loxP sites and deletion of exon 4 by Cre-recombinases created a frameshift in downstream exons, generating an early STOP codon. (B) Immunoblotting of total striatal lysates from WT, D1-KO, and D2-KO mice. Representative immunoblot images of WAVE1 and actin (Upper) and quantified WAVE1 levels normalized to actin levels are shown. Data represent means ± SEM compared with WT. n = 4 per group. **P < 0.01 and ***P < 0.001, t test. (C) Immunohistochemically stained WAVE1 in the striatum of WT mice harboring the Drd1a-Cre allele or WAVE1 D1-KO mice bred with a Cre-dependent tdTomato reporter line. Arrows and arrowheads indicate D1-MSNs and D2-MSNs, respectively. (Scale bars, 10 µm.)