Fig. S5.

Effect of acute challenge of cocaine after withdrawal on dendritic spine density in the NAc shell. WT mice (A) and WAVE1 D1-KO mice (B) were injected daily with cocaine (15 mg/kg) for 14 d. Mice were subsequently subjected to 14 d of withdrawal and then acutely challenged with saline or cocaine (15 mg/kg). One hour after the last injection, mice were perfused and dendritic spines were analyzed in DiI-labeled D1-MSNs in the NAc shell. D1-MSNs was identified as enkephalin⁻. (Upper panels) Representative images of dendritic segments. (Scale bars, 5 µm.) (Lower graphs) Quantification of total and four classes of dendritic protrusion density. Data represent means ± SEM. *P < 0.05, Kolmogorov–Smirnov test. n = 15–17 mice per group. The total numbers of dendrites analyzed were 30 (WT-Sal), 30 (WT-Coc), 32 (KO-Sal), and 34 (KO-Coc).