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. 2017 Jan 23;114(6):E1027–E1035. doi: 10.1073/pnas.1615123114

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Fig. 4. — Rescue of hybrid seeds involving tetraploid A. lyrata is associated with restored endosperm cellularization. Feulgen-stained seeds of tetraploid A. lyrata, A. arenosa, and interploidy interspecific hybrids are depicted as follow: (A and B) 4x A. arenosa at 9 and 15 DAP, respectively. (C and D) 4x A. lyrata at 9 and 15 DAP, respectively. (E and F) 4x A. lyrata × 2x A. arenosa at 9 and 15 DAP, respectively. (G and H) 2x A. arenosa × 4x A. lyrata at 9 and 15 DAP, respectively. (I and J) 4x A. arenosa × 2x A. lyrata at 9 and 15 DAP, respectively. (K and L) 2x A. lyrata × 4x A. arenosa at 9 and 15 DAP, respectively. (M and N) 4x A. arenosa × 4x A. lyrata at 9 and 15 DAP, respectively. (O and P) 4x A. lyrata × 4x A. arenosa at 9 and 15 DAP, respectively. The directionality of the cross is indicated seed parent (♀) × pollen donor (♂). An early (9 DAP) and late (15 DAP) developmental time point is displayed. Images shown in this figure are representative for a total number of observed seeds of at least 20 per biological replicate (three replicates). (Scale bar, 50 µm.) 2A: 2x A. arenosa; 2L: 2x A. lyrata; 4A: 4x A. arenosa; 4L: 4x A. lyrata.