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. 2017 Jan 23;114(6):E1027–E1035. doi: 10.1073/pnas.1615123114

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Fig. 5. — Rescue of hybrid seeds involving tetraploid A. lyrata does not correlate with endosperm proliferation rate. (A) Endosperm proliferation was assessed by counting endosperm nuclei number at 5, 7, and 9 DAP. (B) Proliferation rate was calculated as the ratio of nuclei number at 5 and 7 DAP and at 7 and 9 DAP. Parental tetraploid species are represented by dark red (A. arenosa) and dark blue (A. lyrata) colors and reciprocal hybrids by shades: from darker to lighter red, 4x A. arenosa × 2x A. lyrata, 2x A. lyrata × 4x A. arenosa and 4x A. arenosa × 4x A. lyrata respectively; from darker to lighter blue, 4x A. lyrata × 2x A. arenosa, 2x A. arenosa × 4x A. lyrata, 4x A. lyrata × 4x A. arenosa. The cross (†) at 9 DAP indicates no or few endosperm nuclei detectable in 4x A. arenosa × 2x A. lyrata hybrid seeds. In A, error bars represent SD between three biological replicates (n = 5 seeds per sample). In B, the error bars were calculated as follow: ratio value × [(coefficient of variation_time2²)+(coefficient of variation_time1²)]/2. 2A: 2x A. arenosa; 2L: 2x A. lyrata; 4A: 4x A. arenosa; 4L: 4x A. lyrata.