Fig. S8.
Ploidy level assessment of the synthetic 4x A. lyrata and 4x A. arenosa. Ploidy levels were estimated by measuring the relative nuclear DNA content of each sample by flow cytometry. The A. lyrata and A. arenosa diploid controls are shown in A and B. On top of nuclear DNA, content matching with diploid cells (2C), tetraploid (4C), and octoploid (8C) cells can be seen because of different mitotic stages and endoreduplication. The relative nuclear DNA content of colchicine treated positive stems (4x) is shown for A. lyrata (C) and A. arenosa (D). In these samples, as in diploids, an octoploid level (8C) is seen because of cells in G2 mitotic phases and endoreduplication, but tetraploidy (4C) is the lowest ploidy level observable. Progenies obtained from synthetic 4x A. lyrata × 2x A. arenosa (E) and 2x A. arenosa × synthetic 4x A. lyrata (F) are triploid as shown by the intermediate relative nuclear DNA content between diploids and tetraploids (3C, 6C, and 12C). Progenies obtained from synthetic 4x A. arenosa × synthetic 4x A. lyrata (G) are tetraploid, as their relative nuclear DNA content was similar to the tetraploid parents. For each biological sample, n = 5 replicates. The directionality of the cross is indicated seed parent (♀) × pollen donor (♂). 2A: 2x A. arenosa; 2L: 2x A. lyrata ; 4Asynth: synthetic 4x A. arenosa; 4Lsynth: synthetic 4x A. lyrata.