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. 1991 Dec 15;88(24):11071–11075. doi: 10.1073/pnas.88.24.11071

Bag model for DNA migration during pulsed-field electrophoresis.

G Chu 1
PMCID: PMC53075  PMID: 1763022

Abstract

A model for pulsed-field electrophoresis was developed by picturing large DNA as a deformable "bag" that (i) moves with limiting mobility in a continuous electric field, (ii) adopts an orientation aligned with the field direction, and (iii) reorients after a change in field direction in a size-dependent manner. The model correctly predicted the resolution of large DNA in a pulsed field including the surprising phenomena of mobility inversion, lateral band spreading, and improved resolution for obtuse angles. A simple parametrization agreed with observations of two completely different aspects of DNA behavior: bulk mobility as measured during gel electrophoresis and molecular reorientation as measured by linear dichroism. The model also provides quantitative guidelines for setting experimental parameters in pulsed-field electrophoresis experiments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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