Table 2.

Enterococcus virulence genes and PCR primers which were used in this study

Virulence factor	Gene	Localisation	Primer name	Oligonucleotide sequence (5’ to 3’)	Product size	Primer source
Cytolysin	cylA	Chromosome/Plasmide	CYT I CYT IIb	ACTCGGGGATTGATAGGC GCTGCTAAAGCTGCGCTT	688	Vankerckhoven et al., 2004 [40]
Enterococcal surface protein	esp	Chromosome	ESP 14 F ESP 12R	AGATTTCATCTTTGATTCTTGG AATTGATTCTTTAGCATCTGG	510	Vankerckhoven et al., 2004 [40]
Hyaluronidase	hyl	Chromosome	HYL n1 HYL n2	ACAGAAGAGCTGCAGGAAATG GACTGACGTCCAAGTTTCCAA	276	Vankerckhoven et al., 2004 [40]
Aggregation substance	asa1	Plasmide	ASA 11 ASA 12	GCACGCTATTACGAACTATGA TAAGAAAGAACATCACCACGA	375	Vankerckhoven et al., 2004 [40]
Gelatinase	gelE	Chromosome	GEL11 GEL12	TATGACAATGCTTTTTGGGAT AGATGCACCCGAAATAATATA	213	Vankerckhoven et al., 2004 [40]
Cell wall adhesin of E. faecium	efaAfm	Chromosome	TE37 TE38	AACAGATCCGCATGAATA CATTTCATCATCTGATAGTA	735	Reviriego et al., 2005 [41]
Cell wall adhesin of E. faecalis	efaAfs	Chromosome	TE5 TE6	GACAGACCCTCACGAATA AGTTCATCATGCTGTAGTA	705	Reviriego et al., 2005 [41]
Sex pheromone	ccf	Chromosome	TE53 TE54	GGGAATTGAGTAGTGAAGAAG AGCCGCTAAAATCGGTAAAAT	543	Reviriego et al., 2005 [41]