Fig. 1.

Tacrolimus restored glucose tolerance in the chronically overfed HFD-dNONcNZO dams. A–C Oral glucose tolerance test (OGTT) performed prior to treatment with tacrolimus (A) and after 2 weeks (B) or 4 weeks (c) of tacrolimus (0.1 mg/kg). D–F Effect of HFD chronicity and glycemic response to metformin or tacrolimus in the HFD-dNONcNZO mice chronically fed with 60% kcal % HFD for 7 weeks (D), 15 weeks (E) or 25 consecutive weeks (F), respectively. Representative OGTTs were from two sets of experiments (n = 5–8/group) analyzed with one-way ANOVA followed by Dunn’s multiple post hoc test comparing treated mice to HFD-dNONcNZO