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. 2017 Feb 13;14:28. doi: 10.1186/s12985-017-0685-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 11 — Western blot analysis of DENV 2 structural and non-structural proteins. HEK293T/17 cells were treated with the concentrations of orlistat as indicated or not treated (DENV) and subsequently mock infected (mock) or infected with DENV 2. At 24 h.p.i the expression of (a) DENV 2 E and NS3 proteins and (d) FASN was determined by western blotting. All filters were stripped and re-probed with actin as a loading control. Experiments were undertaken independently in triplicate and signals quantitated (b, c, e). Bars show mean +/−SD (*; p value <0.05)